Journal: Molecular cell

Article Title: HOTTIP -dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia

doi: 10.1016/j.molcel.2022.01.014

Figure Lengend Snippet: Key resources table

Article Snippet: For generation of the catalytically dead RNaseH-fused dCas9, mutated RNaseH (D210N) (Addgene #111904) was subcloned into the pHR-dCas9 vector (Addgene #46911) as a Sbf1-BamHI restriction fragment. sgRNA targeting the CTNNB1 CBS-u2 was subcloned into the pLKO5.sgRNA.EFS.tRFP vector (Addgene #57824) and transduced along with the dCas9-RNaseH fusion vector into AML cells, and then GFP/RFP double positive cells were selected by FACS.

Techniques: Recombinant, CRISPR, Electroporation, Protease Inhibitor, Staining, Transfection, Purification, Plasmid Preparation, Multiplex Assay, Hybridization, Sensitive Assay, SYBR Green Assay, Transgenic Assay, Software

Journal: microPublication Biology

Article Title: An assessment of genome-editing efficiency of a newly developed Cas9 in C. elegans

doi: 10.17912/micropub.biology.000601

Figure Lengend Snippet:

Article Snippet: pDD162 , P eft-3::Cas9::tbb-2 3’UTR; P U6::empty sgRNA , Addgene #47549.

Techniques: Plasmid Preparation